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The selection of high-yield Alkaline protease strains

Zhu Bao-cheng

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Abstract

AbstrcatA mutant strain H26 producing alkaline protease was obtained from the initial strain Bacillus licheniformis XP2,treated with ~60Co,NTG and repeated heating. This mutant strain showed good genetic stability in the procedure of continual inoculation and preservation. This strain can produce 32000u/ml alkaline protease. Compared with that of the initial strain, the enzyme productivity of the strain H26 has increased by 68% on the industrial production scale.

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What this paper is about

AbstrcatA mutant strain H26 producing alkaline protease was obtained from the initial strain Bacillus licheniformis XP2,treated with ~60Co,NTG and repeated heating. This mutant strain showed good genetic stability in the procedure of continual inoculation and preservation. This strain can produce 32000u/ml alkaline protease. Compared with that of the initial strain, the enzyme productivity of the strain H26 has increased by 68% on the industrial production scale.

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Available abstract

AbstrcatA mutant strain H26 producing alkaline protease was obtained from the initial strain Bacillus licheniformis XP2,treated with ~60Co,NTG and repeated heating. This mutant strain showed good genetic stability in the procedure of continual inoculation and preservation. This strain can produce 32000u/ml alkaline protease. Compared with that of the initial strain, the enzyme productivity of the strain H26 has increased by 68% on the industrial production scale.

Key concepts: Strain (injury), Bacillus licheniformis, Protease, Enzyme, Mutant, Yield (engineering), Alkaline protease, Biology

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