Selection of SSR Core Primers and Varietals Purity Identification in Eggplant

To establish a fast and accurate hybrid identification method using molecular markers urgently.236 pairs of SSR markers were used for polymorphic markers selection in three eggplant varieties,and purity identification was conducted in both laboratory and field in order to get finger printing information.The results showed that ultimately 15 pairs of polymorphic primers used in purity identification was selected.Application of these primers,variety 1-2010,3-2010 and 9-2010 purity results were 98%,96% and 100%,finger printing information was achieved on this basis.Field test results consistent with laboratory ones,it identified purity of eggplant hybrids using molecular markers was feasible and efficient,which has a certain theoretical and practical values.