Studies on Identifying Authenticity and Purity of Corn Variety by Using SSR Markers

[Objective] The study was to discuss the interpretation standards about molecular detection on authenticity and purity in the seeds of the hybrid maize and its parents.[Method] With 16 hybrids and their parents and 202 skeleton inbred lines as the primers for screening materials,the artificial groups of 3 hybrids and 3 inbred lines were build resp.as the verification materials.Genomic DNA of seedlings were extracted by using CTAB method.137 pairs of SSR primers were selected for SSR amplification and product detection,which was used in the authenticity identification and purity detection of maize varieties.The results was compared with the planting identification results of the same batch materials transplanted into the field.[Result] 20 pairs of first-level and 20 pairs pof secondary-level core primers with high polymorphism information content(PIC value),clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers.The authenticity of hybrid artificial groups was identified with first-level core primers.According to the amplification results,3 pairs(phi080,umc1196,umc2084) of primers with good performance were selected for the purity detection of artificial groups of hybrids.The identification results using SSR molecular had a high degree of consistency with the results of the same batch materials planted in the field.[Conclusion] The method of using SSR markers to identify the authenticity and purity of maize varieties was feasible.