Cloning of Pseudorrabies Virus Fa Strain gE Gene and Comparative gE Sequence of Five PRV Virus Strains

By means of polymerase chain reaction,about 17 kb fragment was amplified from PRV Fa strain.The PCR product was proved to be true by NcoⅠ?SmaⅠ and SphⅠ restriction enzyme analysis,and then cloned into PUC18 plasmid.Through restriction enzyme digestion and Dotblot,the recombinant PpgE plasmid was indicated containing gE gene.Sequencing of gE gene in PUC18 plasmid,Comparative gE sequence of five PRV virus strains,the homologue among these virus strains was at least 96%.Therefore it was proved that gE gene is a conservative gene.Taiwan strain was higher homologue with China strains in gE gene.Nucleotides 10371407 sits were identical in all strains.It provided a useful PCR region or probe to detect the latency of PRV.In addition,this reaseach was a foundation to get gE proteins in vitro.