Construction of Prokaryotic Expression Plasmid of gE Gene of PRV GZ-Z1 Strain

The gE gene was amplified by PCR technology taking DNA of PRV GZ-Z1 strain as the template based on a pairs of specific primers.Then the amplified product was linked with pMD18-T vector.Finally,the identified amplified product was cloned into the pET32a(+) prokaryotic expression vector.The sequencing results showed that the gE gene with an intact open reading frame,1734bp and 577 amino acids was in the pET32a(+) vector correctly,which indicated that the pET32a-gE prokaryotic expression plasmid was structured successfully.The homology of amino acid sequence between gE gene and other18 PRV strains from home and abroad was 94.8%～98.6% and the gE gene of PRV GZ-Z1 strain and foreign isolated strains belonged to the same evolution branch.The relationship between the gE gene of PRV GZ-Z1 strain and domestic Fa,Min and GDSH strains was very far.