2007•Anhui nongye kexueRequires access
Establishment of Regeneration System from Cotyledon Explant of Pepper and Screening Test for its Resistance to Kanamycin
Liping Sun
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Abstract
With Zhongjiao No.2 pepper as experimental material,an efficient regeneration system from cotyledon explant of pepper and screening test for its resistance to Kanamycin was established.The results showed that the optimal medium for bud differentiation was MS+BA 5.0 mg/L+IAA 0.5 mg/L+AgNO3 6.0 mg/L,the optimal medium for bud elongation was MS+BA 5.0 mg/L+IAA 0.5 mg/L+GA3 2.0 mg/L+AgNO3 6.0 mg/L,the optimal medium for root inducing was MS+IAA 1.0 mg/L,and the eventual concentration of Kanamycin for selecting was 50 mg/L.