Submerged culture of Agaricus blazei Murill

By single factor experiments,submerged culture of Agaricus blazei Murill was studied.Plackett-Burman experiment was carried out,and the results were calculated in Minitab 15 software,in which four important factors were shown as follows: the concentration of sucrose and yeast extract,temperature,and microelement ratio.The four important factors were optimized by the orthogonal experiment design.The results showed that under the conditions of sucrose 45 g/L,yeast extract 3 g/L,KH2PO4 2.5 g/L,MgSO4 1.25 g/L,temperature 27 ℃,the yield of polysaccharide reached 4.64 g/L after 7 d culture.