Evaluation of Transfer Traceability for Automated Hematology Analyzers with Fresh Whole Blood

Objective To calibrate hematology analyzers with healthy fresh blood to make their parameters comparable and traceable.Methods Hematology analyzer Sysmex XE-2100 was adopted as the reference system to measure fresh blood samples collected from healthy subjects.The detected results of white blood cell(WBC),red blood cell(RBC),hemoglobin(Hb),hematocrit(HCT) and platelet(PLT) were used to calibrate hematology analyzer Sysmex XS-1000i,and comparison studies were performed between two analyzers before and after calibration.Results Before Sysmex XS-1000i was calibrated,the deviation arising in WBC counting displayed on Sysmex XS-1000i was beyond the limits;after it was calibrated,the deviations of all the parameters shown on Sysmex XS-1000i were within the limits.Conclusions Parameters of hematology analyzers calibrated with fresh blood are comparable and traceable.