Antibiotic resistance of bacteria in ground water body of Binzhou

Objective To survey the condition of antibiotic resistance in ground water body of Binzhou,and to study its relationship with plasmid profiles.Methods Water samples were collected from 4 places of ground water body of Binzhou,then CFU was counted and the antibiotic resistance rate was analyzed.K-B method was used to practice the susceptibility test with 5 antibiotics,then the antibiotic resistance spectrums were analyzed.Results The ampicillin resistance rate of the samples varies from 11.7% to 49.8%.The ampicillin resistant isolates have different antibiotic resistance rate which varies from 12.5% to 25% relevant to 3 other antibiotics,and the cilastain resistance was not found.Isolates with the trait of ampicillin resistance and one non-β-lactam antibiotic reached 32.5%,and isolates with the trait of resistant to ampcillin and two or more non-β-lactam antibiotics reached 20%.There are 63 plasmids bands which varys from 815bp to 12.2kb in plasmid profiles.Conclusion The antibiotic resistance in ground water body of Binzhou was prevalent,and the phenomenon of multidrug resistance is obvious too.It is significant and necessary to monitor the antibiotic resistance status.