Research of Moxibustion's Effect on Regulation of Colonic Fibroblasts Proliferation in Ulcerative Colitis Fibrosis Rats

Objective To investigate the mechanisms of moxibustion in the treatment of the colonic fibrosis in ulcerative colitis(UC) by observing the colonic fibroblast(CFB) proliferation in ulcerative colitis fibrosis rats.Methods A rat model of ulcerative colitis fibrosis was established by immunological methods using human colonic mucosa as antigen adding local stimulation.The rats were randomly divided into normal group,model group,herb-partition moxibustion group,mild-warm moxibustion group and western medicine group(SASP group).Herb-partitioned moxibustion group and mild-warm moxibustion group treated by herb-partitioned moxibustion and mild-warm moxibustion respectively on Qihai(CV 6) and Tianshu(ST 25,bilateral) points.SASP group fed with salicylazosulfapyridine.Colonic fibroblasts from all the rats were isolated and cultured and the proliferation of colonic fibroblasts was determined by flow cytometry.Results Colonic fibroblasts from ulcerative colitis fibrosis rats' conlon proliferated faster than those of normal ones.The Colonic fibroblasts number in model group in G0-G1 period decreased(P0.01),in G2-M period and S period increased(P(0.01)).Treatment of herbs-partition moxibution and mild-warm moxibution can inhibit its proliferative index.Conclusion Moxibution on Qihai(CV 6) and Tianshu(ST 25,bilateral) points is effective in preventing the over proliferated of colonic fibrosis in ulcerative colitis fibrosis rats.