Optimization of Saccharomyces cerevisiae medium with molasses as carbon source

Using beet molasses as carbon source, based on shake flask test, on the basis of single factor experiments, orthogonal experiment was adopted to optimize the formula of Saccharomyces cerevisiae medium. The optimum formula was determined as follows: beet molasse 8%, urea 0.05%, monopotassium phosphate 0.05%. Under the optimized condition, the cell weight reached 7.67 g/l, which was 23.3% higher than the non-optimized one.