Influence of main components in the medium on biomass of selected wine yeast

Based on shake flask results and in orderto improve the biomass as much as possible,the main components in the medium,which affect the biomass of Saccharomyces cerevisia LFN518,were optimized by the combination of single factorexperiment and response surface methodology.The related mathematical model was eastablished.The optimal medium was obtained by response surface analysis as maltose 31.6 g/L,yeast extract 18.1 g/L,potassium hydrogen phosphate 8.0 g/L,magnesium sulfate 12.0 g/L,calcium chloride 6.0 g/L and vitamin mother liquor 6.0 ml/L.The biomass of Saccharomyces cerevisiae grew on optimal medium was 8.124 g/L,which was 29.984%higherthan that before optimization(6.25 g/L).