Effect of Jiangzhi Mixture on LDL Susceptibility to Oxidation and Liver Cells LDL-R Gene Expression of Hyperlipidemia Rats

Objective:To study the effect of Jiangzhi Mixture(IM) on low density lipoprotein(LDL) oxidative susceptibility and low density lipoprotein receptor(LDL-R) gene expression in rats with hyperlipidemia,and elucidate the possible mechanism of IM regulating blood lipid.Method: Sixty Wistar male rats were randomly divided into normal group,model group,simvastatin and IM high,middle,low dose groups,10 rats in each group.The rats in normal control group were fed with basal diet,the rats in the other groups were fed with a high fat diet to establish hyperlipidemia rat models,and during modeling the drugs were used for prevention.Physiological saline were given to the rats in control group and model group,simvastatin group rats were given 7.2×10-4g · kg-1 simvastatin suspension solution,and Jiangzhi Mixture low,middle,and high dose groups were intragastrically given 0.25,0.5,1 g · kg-1decoction,respectively,10 mL · kg-1,once a day for 10 weeks,during making model.After 10 weeks,according to the method of Abstracting eyeball blood,the blood lipid spectrum of each individual animal in various groups were measured with full automatic biochemical analyzer.After separation of LDL,its susceptibility to oxidation was determinated in vitro.According to the method of Trizol extracting liver RNA of rats,LDL-R mRNA expression was determinated by RT-PCR.Result: Compared with model group,the cholesterol(TC),triglycerides(TG),low density lipoprotein chelesterol(LDL-C),and apolipoprotein B(Apo B) levels in Jiangzhi Mixture groups were regulated and inhibited;the high density lipoprotein chelesterol(HDL-C),apolipoprotein A(Apo A) levels and Apo A/Apo B ratio were increased,meanwhile the differences were statistically significant(P0.05 or P0.01);Compared with the model group,the maximum rate of oxidation time(Tmax) and LDL oxidation lag time(lag time) were significantly prolonged,and the relative expression levels of liver LDL-R mRNA were significantly elevated in all Jiangzhi mixture dose groups,and the differences were statistically significant(P0.05 or P0.01).Conclusion: Jiangzhi Mixture can modulate blood lipid metabolism in experimental hyperlipidemia rats models,reduce LDL susceptibility to oxidation of hyperlipidemia rat significantly,and induce liver tissue LDL-R mRNA expression of hyperlipidemia rat,and have good adjusting effect on lipid abnormalities.