Screening specific serum biomarkers of 2616 pregnant women during the second trimester

Objective Toevaluatetheefficiencyof screening specific biomarkers inthesecond-trimester pregnant women.Methods Serum levels ofAFP、β-HCG and uE3 were measured by CLIAor ELISAin 2616 pregnant women during 14～24 weeks, and the risk rate was analyzed with the T21﹡SOFTw program, the high-risk pregnant women were proposed to take fetal amniotic fluid or high resolution ultrasound examination.Results The high-risk rates for Down's syndrome, 18-trisomy syndrome and neural tube defects were 5.73%, 0.34%, 1.11% respectively;there were one Down's syndrome and two neural tube defects, three other chromosomal abnormalities by prenatal diagnosis.Conclusion Screening analysis of second-trimester pregnancy serum is a major to prevent chromosomal disease and neural tube defects;the precise gestational age is the most important factor in the screening software.