Molecular Cloning and Sequence Analysis of Ovine TTF-1 Gene

Taking total DNAs from blood of Small Tail Han sheep as template, ovine thyroid transcription factor 1 (TTF-1) was amplified by specific primers (P1 and P2). Then pGEM-T vector was cloned into, positive clones were screened and digested by restriction endonuclease after transforming, and TTF-1 gene sequence and its deduced amino acid sequence were analyzed. The results showed that the sequence length of the TTF-1 gene was 1 459 bp, encoded for 174 amino acids including parts of Exon 1 and Exon 2 seguences and one intron. The exon sequences of TTF-1 gene shared 98.7%,98.3%,97.5%,96.6%,96.6% homology with the published genomic sequences of dog, pig, cattle, human and mouse. And the intron sequences of TTF-1 gene shared 83.7%, 74.9%, 59.5% homology with the published genomic sequences of pig, human and mouse.