2012•Hubei nongye kexueRequires access
Optimization of Enzymatic Hydrolysis Conditions of Peanut Meal
Ganwei Zhang
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Abstract
Peanut meal was hydrolyzed with alcalase alkaline protease.Effects of reaction time,mass ratio of peanut meal,dose of enzyme,reaction temperature and pH on hydrolysis degree of peanut meal protein were studied by single factor tests and orthogonal experiment.The results showed that the optimum hydrolysis conditions were as follows,reaction temperature,60 ℃;pH 8.5;mass ratio of peanut meal,5%;enzyme dose,7%;and reaction time,4 h.Under these conditions,the hydrolysis degree of peanut meal protein was 29.18%.