Directly enzymatic hydrolysis of peanut meal

Peanut meal was hydrolyzed with Alcalase alkaline protease to make peanut protein hydrolyzate.The influence factors,such as time,temperature,pH value,concentration of substrate and the amount of enzyme were studied.The hydrolysis degree and recovery rate of nitrogen were used as indexes,and the optimum hydrolysis conditions were determined through the single factor test and orthogonal experiment as follows:mass concentration of substrate 30 g/L,pH 9.5,temperature 60℃,the amount of enzyme 7%,time 4 h.The crude product was decolorized,and the optimum conditions for decolorization were as follows:the amount of active carbon 3%,pH 4,temperature 60℃,time 40 min.Under the optimum conditions,the degree of hydrolysis was 27.81% and nitrogen recovery rate was 70.01%.