Study on the extraction of active protein in rape pollen

Rape pollen which was stored more than 1 year was used as material in this study. The effect of temperature difference cell-fragmentated method was compared with hydrolization with enzymes from juice of garlic. A research on protein fractionation of rape pollen defatted and cell-fragmentated and their antioxidant effect were performed. On the basis of study,the optimum conditions of extraction of globulins was gained. The results showed that the temperature difference was best choice,glutelins and globubins were found to be the predominant proteins in rape pollen. Through the orthogonal test,the optimum conditions of extraction have been obtained as follows: nacl concentration 0.20mol/L, pH value 5, time 6h, times 3, temperature 4℃.