Effects of nm23-h1 on cell proliferation and PKC-α expression in oral squamous cell carcinoma

Objective To study the effects of nm23-h1 on cell proliferation and possible mechanism in oral squamous cell carcinoma.Methods The expression of nm23-h1 was detected in six oral squamous cell carcinoma cell lines.The cell line of the lowest nm23-h1 expression with highest transfection efficiency was used to transfect nm23-h1 instantaneonsly.Cell proliferation was detected by the method of MTT and the expression of PKC-α examined by realtime PCR.Results High metastatic cell lines Tb and Tca8113M expressed the lowest nm23-h1 in six oral squamous cell carcinoma cell lines which involved Tca8113,Tb,Tca8113M,TSCC,OSC-4,Tca8113/CDDP.Attenuated cell proliferation and higher expression of PKC-α were oberseved in transfected Tb cells.Conclusion Transfection of nm23-h1 attenuated cell proliferation of Tb and PKC-α signaling might be involved in this process.