2007•China Journal of Oral and Maxillofacial SurgeryRequires access
Expression of CXCR4 and its modulation on migration,proliferation of oral squamous cell carcinoma cell line
Xinming Chen
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Abstract
PURPOSE: This study was aimed to evaluate the expression of chemokine receptors CXCR4 in oral squamous cell carcinoma cell line and to determine the role of CXCR4 in tumor cell proliferation and migration. METHODS: The expression of CXCR4 in oral squamous cell carcinoma cell line was evaluated by immunofluorescent staining and flow cytometry.MTT assay was used to determine the SDF-1/CXCR4 role on proliferation of tumor cells. Cell migration assays were carried out with the oral squamous cell carcinoma cell line to confirm the effect of SDF-1/CXCR4 on migration. SPSS 10.0 software package was used for ANOVA and Student's t test. RESULTS: Oral squamous cell carcinoma cell line displayed the CXCR4 expression with great intensity and the expression rate was 68.62%. In MTT assay,recombinant SDF-1 stimulated proliferation of OSCC cell and CXCR4 neutralization by monoclonal antibodies decreased proliferation. In migration assay,SDF-1 induced migration of OSCC cells. The migration cell mean of SDF-1 stimulated group was significantly higher than the control group (P0.05). CONCLUSION: The expression of CXCR4 was significantly correlated with oral squamous cell carcinoma cell proliferation and migration.