Optimization of Extraction Conditions for Proanthocyanidins from Grape Seeds by Accelerated Solvent Extraction

Response surface methodology was applied to optimize the extraction of proanthocyanidins from seeds of the new grape cultivar Meili using an accelerated solvent extraction(ASE) system.The optimal extraction conditions were found to be 13 min,1161 psi,80% and 80 ℃ for extraction time,pressure,methanol concentration and temperature,respectively.The maximum predicted extraction yield of proanthocyanidins under the optimized conditions was 6.817% indicating a relative error of 7.79% as compared with the actual value of 6.286% obtained after the third repeated extraction.The purity of proanthocyanidins under the optimized conditions was 41.02%.