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2003•Chinese Journal of Hospital PharmacyRequires access

Sampling on paper-HPLC determination of amino acids in whole blood

Wen Luo

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Abstract

OBJECTIVE To develop a quantitive method with sampling on paper-HPLC for the determination of six amino acids in whole blood.METHODS Small amount of whole blood was absorbed on a piece of filter paper which was derivatized with 2,4-dinitro-1-fluorobezene,and analyzed with HPLC.RESULTS The method was linear within range of 0.5 ～10 mg·L -1 ,and the average recoveries for each of the six amino acids were Val: 99.0% 、Met: 90.5% 、Ile: 95.1% 、Leu: 97.4% 、Phe: 94.6% 、Tye: 96.6% ,respectively.CONCLUSIONS The method is easy to perform for the determination of blood amino acid concentration of the newborn and children.

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OBJECTIVE To develop a quantitive method with sampling on paper-HPLC for the determination of six amino acids in whole blood.METHODS Small amount of whole blood was absorbed on a piece of filter paper which was derivatized with 2,4-dinitro-1-fluorobezene,and analyzed with HPLC.RESULTS The method was linear within range of 0.5 ～10 mg·L -1 ,and the average recoveries for each of the six amino acids were Val: 99.0% 、Met: 90.5% 、Ile: 95.1% 、Leu: 97.4% 、Phe: 94.6% 、Tye: 96.6% ,respectively.CONCLUSIONS The method is easy to perform for the determination of blood amino acid concentration of the newborn and children.

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Available abstract

OBJECTIVE To develop a quantitive method with sampling on paper-HPLC for the determination of six amino acids in whole blood.METHODS Small amount of whole blood was absorbed on a piece of filter paper which was derivatized with 2,4-dinitro-1-fluorobezene,and analyzed with HPLC.RESULTS The method was linear within range of 0.5 ～10 mg·L -1 ,and the average recoveries for each of the six amino acids were Val: 99.0% 、Met: 90.5% 、Ile: 95.1% 、Leu: 97.4% 、Phe: 94.6% 、Tye: 96.6% ,respectively.CONCLUSIONS The method is easy to perform for the determination of blood amino acid concentration of the newborn and children.

Key concepts: High-performance liquid chromatography, Chromatography, Amino acid, Whole blood, Blood sampling, Chemistry, Sampling (signal processing), Filter paper

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