Effects of IL-1α secreted from keratinocytes on fibroblasts

Objective To observe the effects of IL-1α derived from keratinocytes on proliferation and collagen secretion of fibroblasts. Methods The immunohistochemical method was employed to confirm whether the keratinocytes can secrete IL-1α.Conditioned medium of keratinocytes with different concentration of IL-1α antibody was added to the fibroblasts,Samples were divided into the tested groups(0.04μg/ml,0.2μg/ml,1μg/ml group)and the control(0μg/ml group). The CCK-8, radio-immunity methods were employed to measure the cell proliferation and collagen secretion. Results Keratinocytes did secret IL-1α. In fibroblast proliferation, absorbency (A value) of tested groups was different from the control's (P0.01). A value decreased as the concentration increasing(P0.01),the difference between 0.2μg/ml group, 1μg/ml group and 0.04μg/ml group had statistic meaning, but the difference between 0.2μg/ml group and 1μg/ml group had no statistic meaning. In collagen secretion, the concentration of Ⅲ procollagen increased as the concentration increasing(P0.01),the difference between 0.2μg/ml group, 1μg/ml group and 0.04μg/ml group had statistic meaning, but the difference between 0.2μg/ml group and 1μg/ml group had no statistic meaning. Conclusion keratinocytes can secret IL-1α which can increase fibroblasts proliferation ,inhibit collagen sercetion .