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Immunophenotyping analysis of newly diagnosed childhood acute lymphoblastic leukemia by three-color flow cytometry

Hua Jin

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Abstract

Objective:To study the character of immunophenotype s in childhood acute lymphoblastic leukemia(ALL) using three-color flow cytometry.Methods:Immunophenotyping assay for 37 cases of childhood ALL was performed by FCM analysis using CD 45 /SSC gating.Results:Among 37 cases of ALL,34 cases (91.9%) were B-ALL,in which the expression of CD 19 was 100%,much higher than those of CD 10 (82.4%,P0.05) and CD 22 (61.8%,P0.01).Twenty-seven percent of all patients was expressed with myeloid-associated antigen mainly including CD 33 .Conclusion:B-ALL is the major subtype in childhood ALL.CD 19 ,which is expressed stabley in B lineage ALL,is reliable cell membrane marker for the diagnosis of B-ALL.Relatively higher expression of CD 34 and myeloid-associated antighen were observed in childhood ALL.

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What this paper is about

Objective:To study the character of immunophenotype s in childhood acute lymphoblastic leukemia(ALL) using three-color flow cytometry.Methods:Immunophenotyping assay for 37 cases of childhood ALL was performed by FCM analysis using CD 45 /SSC gating.Results:Among 37 cases of ALL,34 cases (91.9%) were B-ALL,in which the expression of CD 19 was 100%,much higher than those of CD 10 (82.4%,P0.05) and CD 22 (61.8%,P0.01).Twenty-seven percent of all patients was expressed with myeloid-associated antigen mainly including CD 33 .Conclusion:B-ALL is the major subtype in childhood ALL.CD 19 ,which is expressed stabley in B lineage ALL,is reliable cell membrane marker for the diagnosis of B-ALL.Relatively higher expression of CD 34 and myeloid-associated antighen were observed in childhood ALL.

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Available abstract

Objective:To study the character of immunophenotype s in childhood acute lymphoblastic leukemia(ALL) using three-color flow cytometry.Methods:Immunophenotyping assay for 37 cases of childhood ALL was performed by FCM analysis using CD 45 /SSC gating.Results:Among 37 cases of ALL,34 cases (91.9%) were B-ALL,in which the expression of CD 19 was 100%,much higher than those of CD 10 (82.4%,P0.05) and CD 22 (61.8%,P0.01).Twenty-seven percent of all patients was expressed with myeloid-associated antigen mainly including CD 33 .Conclusion:B-ALL is the major subtype in childhood ALL.CD 19 ,which is expressed stabley in B lineage ALL,is reliable cell membrane marker for the diagnosis of B-ALL.Relatively higher expression of CD 34 and myeloid-associated antighen were observed in childhood ALL.

Key concepts: Immunophenotyping, Flow cytometry, Medicine, Myeloid leukemia, Childhood leukemia, Cytometry, Leukemia, Lymphoblastic Leukemia

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