RAPD Analysis of the Genetic Diversity in Chinese Tea Germplasm
Luo Jun-wu
Abstract
Luo Jun-wu
Abstract
The genetic diversity of the tea germplasm(Camellia Section Thea)in China was studied with RAPD method.135 RAPD bands including 123 polymorphic bands were scored in the 23 tea germplasm tested with 11 primers selected.The average of polymorphism was 91.11%.The results of cluster analysis indicated that the minimum genetic distance(0.1378)was between C.kwangnanica and C.grandibracteata,and the maximum genetic distance(0.8995)between C.pubescens and C.crassicolumna.A dendrogram by unweighted pair group method with arithmetic mean algorithm(UPGMA)analysis was obtained.Taxonomy for Camellia Section Thea was discussed by using the dendrogram.
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The genetic diversity of the tea germplasm(Camellia Section Thea)in China was studied with RAPD method.135 RAPD bands including 123 polymorphic bands were scored in the 23 tea germplasm tested with 11 primers selected.The average of polymorphism was 91.11%.The results of cluster analysis indicated that the minimum genetic distance(0.1378)was between C.kwangnanica and C.grandibracteata,and the maximum genetic distance(0.8995)between C.pubescens and C.crassicolumna.A dendrogram by unweighted pair group method with arithmetic mean algorithm(UPGMA)analysis was obtained.Taxonomy for Camellia Section Thea was discussed by using the dendrogram.
Key concepts: Dendrogram, Germplasm, UPGMA, RAPD, Genetic diversity, Camellia, Biology, Genetic distance