Cloning and Analysis of TLR10 Gene in Yak

The aim of the study was to clone TLR10 gene of yak,carry out bioinformatics analysis,assay gene expression profiling in different tissues and provide the basis for the further study in TLR10 gene of yak.This study designed primers and cloned TLR10 gene from yak spleen tissue as bovine TLR10 gene sequences in the GenBank,and the tissue specificity of TLR10 gene was analyzed by semi-quantitative RTPCR.The results of bioinformatics analysis showed that the length of the cloning TLR10 CDS is 2 328 bp.It encodes a peptide of 776 amino acids whose calculated molecular weight is 196.3ku and PI is 4.94.The results of protein prediction showed that TLR10 protein is hydrophilic.Phylogenetic tree and sequence homology analysis showed that yak has higher homology with Bos taurus(99.4%)and also showed highly homology with cattle,sheep and other mammals.The tissue expression profile showed that the TLR10 gene was expressed in 12 tissues of yak.The results showed the TLR10 gene of yak is highly homological and characterized by stable antigen.