ScholarLens Back to search
2008•Chinese Journal of Hospital PharmacyRequires access

Determination of ursolic acid and oleanolic acid in Eriobotrya japonica by HPLC

Zhang Xiang-fei

Open publisher page 1 citations

Abstract

OBJECTIVE To establish a method for the determination of ursolic acid and oleanolic acid in Eriobotrya japonica by HPLC.METHODSThe determination was carried out on a Lichrospher C18(250 mm× 4.6 mm,5 μm)column with methanol-water-glacial acetic acid-triethylamine(90∶10∶0.03∶0.06)as the mobile phase,and detected at the UV wavelength of 215 nm.RESULTSUA and OA can be well resolved(Rs1.65).The calibration curve was in good linearity over the range of 0.4-4.0 μg.CONCLUSIONThe method is simple,accurate,reproducible and can be used to control the quality of Eriobotrya japonica.

About this research paper

What this paper is about

OBJECTIVE To establish a method for the determination of ursolic acid and oleanolic acid in Eriobotrya japonica by HPLC.METHODSThe determination was carried out on a Lichrospher C18(250 mm× 4.6 mm,5 μm)column with methanol-water-glacial acetic acid-triethylamine(90∶10∶0.03∶0.06)as the mobile phase,and detected at the UV wavelength of 215 nm.RESULTSUA and OA can be well resolved(Rs1.65).The calibration curve was in good linearity over the range of 0.4-4.0 μg.CONCLUSIONThe method is simple,accurate,reproducible and can be used to control the quality of Eriobotrya japonica.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To establish a method for the determination of ursolic acid and oleanolic acid in Eriobotrya japonica by HPLC.METHODSThe determination was carried out on a Lichrospher C18(250 mm× 4.6 mm,5 μm)column with methanol-water-glacial acetic acid-triethylamine(90∶10∶0.03∶0.06)as the mobile phase,and detected at the UV wavelength of 215 nm.RESULTSUA and OA can be well resolved(Rs1.65).The calibration curve was in good linearity over the range of 0.4-4.0 μg.CONCLUSIONThe method is simple,accurate,reproducible and can be used to control the quality of Eriobotrya japonica.

Key concepts: Ursolic acid, Eriobotrya, Oleanolic acid, Chromatography, Chemistry, Japonica, Triethylamine, High-performance liquid chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of ursolic acid and oleanolic acid in Eriobotrya japonica by HPLC — Research Paper | ScholarLens