Detection of IMP-1 drug-resistance gene in Pseudomonas aeruginosa and its drug-resistance analysis

Objective:To detect the IMP-1 drug-resistance gene of Pseudomonas aeruginosa and to analyze its drug-resistance to provide evidence for rational antibiotics use.Methods:PCR was used to determine the genes of IMP-1 and ATB expression was used to identify pathogens and test drug sensitivity.Results:106 strains of Pseudomonas aeruginosa were isolated during 2007-2009.The resistant rates to ceftazidime,imipenem,piperacillin,aztreonam,cefoperazone,cefepime,levofloxacin were as follows:50.9%,39.6%,9.2%,56.6%,57.5%,41.5% and 25.5% respectively.Especially the annual detection rates of imipenem-resistant strain were 16.0%,28.3% and 39.6% from 2007 to 2009 respectively.Conclusion:The majority of metallo-β-lactamases-producing genotype IMP-1 are determined from Pseudomonas aeruginosa in ICU.these stains should be monitored closely in clinical treatment.The proper use of effective antibiotics is the key to control infection and delay bacterial drug-resistance.