Cloning and Characterization of Ubiquitin Extension Protein Gene in Grimmia pilifera

Objective:Cloning the ubiquitin extension protein from Grimmia pilifera for the first time,that was the foundation for further gene function study.Method:Total RNA was extracted from plant.Then reverse transeripted by M-MLV.According to the ubiquitin extension protein EST sequence of Grimmia pilifera to design primer.Using 3 'RACE technology(Rapid Amplification of cDNA End) to amplification.Analysising sequence with the molecular biology software.Result:The full-length cDNA of ubiquitin extension protein gene is 673bp(GenBank Accession No.JQ659260),containing a 471bp open reading frame(ORF),encodes a residue of 156 amino acids.Conclusion:The results by analysising the homology of encoded amino acid sequence show that,the deduced amino acid sequence showed more than 96% similarity with the corresponding ubiquitin extension protein fragment of the Physcomitrella patens,Picea sitchensis,Vitis vinifera,Ricinus communis,Populus trichocarpa,Ageratina adenophora.The sequence information of the fragment indicates that this is the ubiquitin extension protein gene fragment.