2013•Journal of Qiqihar UniversityRequires access
Cloning, expression vector construction of GpPER1 gene from Grimmia pilifera and its sequence analysis
Song Lu
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Abstract
A drought-restance relative gene was cloned from a drought cDNA library of Grimmia pilifera,namely 1-Cys peroxiredoxins gene or GpPER1,GenBank login number is GU989314,The full cDNA length of GpPER1 is 1040 bp,The ORF is 666 bp,encoding 221 amino acid.PCR primer were designed according to the ORF of GpPER1,using RT-PCR method,we amplified GpPER1 gene sequence and construct the expression plasmid pRI 101-GpPER1,for further exploring drought resistant mechanisms of Grimmia pilifera in molecular level,and laid a foundation for finding and exploitation of resistant genes from stress-tolerant plant.