Optimization of soybean isoflavone extaraction from okara by ultrasonic and acid hydrolysis

Objective:In this research,the by-products obtained after extracting oil by enzymatic hydrolysis was studied,aiming at recycling the resources and producing isoflavones.Results:The optimal technological conditions of extracting isoflavone from oil by-products using enzymatic hydrolysis were determined:material fluid ratio was 1:12.54,alcohol concentration was 70.28%,hydrochloric acid concentration was 2.6mol/L,hydration time was 30min,hydration temperature was 30℃.Conclusions:The method of ultrasonic and acid hydrolysis was applied for soybean isoflavone extaraction from okara of enzymatic hydrolysis.The content of soybean isoflavone with acid hydrolysis was 42.55% higher than that with 70% ethanol extraction.