OPTIMIZATION OF MICROWAVE-ASSISTED EXTRACTION CONDITIONS OF MYCELIUM POLYSACCHARIDES FROM GRIFOLA FRONDOSA BY RESPONSE SURFACE METHODOLOGY
Cui Yu-jia
Abstract
Cui Yu-jia
Abstract
On the basis of single factor experiments,we optimized the microwave-assisted extraction conditions of mycelium polysaccharides from Grifola frondosa by response surface methodology(RSM),and compared the results with that of the water extraction method.The results showed that the optimal extraction conditions of microwave-assisted extraction method were as follows: microwave power 570.21 W,extraction time 6.91 min and liquid-to-material ratio 40.29 ∶1(V∶M),extraction twice;the actual extraction rate was 11.30%,which has a small relative error in comparison to the predictive value(11.33%);and the polysaccharide extraction rate of the microwave-assisted extraction method was larger than that of the water extraction method by four times.
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On the basis of single factor experiments,we optimized the microwave-assisted extraction conditions of mycelium polysaccharides from Grifola frondosa by response surface methodology(RSM),and compared the results with that of the water extraction method.The results showed that the optimal extraction conditions of microwave-assisted extraction method were as follows: microwave power 570.21 W,extraction time 6.91 min and liquid-to-material ratio 40.29 ∶1(V∶M),extraction twice;the actual extraction rate was 11.30%,which has a small relative error in comparison to the predictive value(11.33%);and the polysaccharide extraction rate of the microwave-assisted extraction method was larger than that of the water extraction method by four times.
Key concepts: Grifola frondosa, Extraction (chemistry), Response surface methodology, Mycelium, Chromatography, Polysaccharide, Microwave, Microwave power