Anti-Oxidant Activities of Total Flavonoids from Flos Puerariae

In order to evaluate the anti-oxidant activities of total flavonoids from Flos Puerariae in vitro,taking 70% ethanol as solvents,the flavonoids was extracted by microwave treatment from Flos Puerariae.The content of the total flavonoids determined by colorimetry is 4.72%;The anti-oxidant activities of total flavonoids from Flos Puerariae was evaluated by means of the reducing power assay, hydroxyl radicals scavenging activity assay,DPPH assay,superoxide anion free radicals.The results show that the flavonoids from Flos Puerariae has different anti-oxidant activities in reducing power assay and scavenging three free radicals.The effects on scavenging O-2·,DPPH· and ·OH are significant.The EC50 is respectively: 0.095mg/ml(O-2·),0.114mg/ml(DPPH·),0.700mg/ml(·OH).It indicates that the reducing power of the flavonoids from Flos Puerariae is relatively high,and the clearance rates to free radicals are positive proportional to the concentration.