Extraction of Total Flavonoids from FLOS LONICERAE JAPONICAE and Its Oxidation Resistance
Yang Xin-jian
Abstract
Yang Xin-jian
Abstract
[Objective] To research on the oxidation resistance of total flavonoids from FLOS LONICERAE JAPONICAE.[Method] Total flavonoids were extracted from FLOS LONICERAE JAPONICAE by ethanol extraction.The oxidation resistance of total flavonoids was detected by Oven Method;the scavenging effect on hydroxy was detected by H2O2/Fe2+ system method;the scavenging effect on superoxide anion was detected by pyrogallol autoxidation method.[Result] Extracts from FLOS LONICERAE JAPONICAE showed significant inhibitory effects on the oxidation of lard oil;the scavenging rates on hydroxyl and free radical reached 52.59% and 17.12%,respectively.[Conclusion] total flavonoids had significant antioxidant ability,hydroxyl radical scavenging activity and superoxide anion scavenging activity.
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[Objective] To research on the oxidation resistance of total flavonoids from FLOS LONICERAE JAPONICAE.[Method] Total flavonoids were extracted from FLOS LONICERAE JAPONICAE by ethanol extraction.The oxidation resistance of total flavonoids was detected by Oven Method;the scavenging effect on hydroxy was detected by H2O2/Fe2+ system method;the scavenging effect on superoxide anion was detected by pyrogallol autoxidation method.[Result] Extracts from FLOS LONICERAE JAPONICAE showed significant inhibitory effects on the oxidation of lard oil;the scavenging rates on hydroxyl and free radical reached 52.59% and 17.12%,respectively.[Conclusion] total flavonoids had significant antioxidant ability,hydroxyl radical scavenging activity and superoxide anion scavenging activity.
Key concepts: Flos, Chemistry, Pyrogallol, Autoxidation, Superoxide, Antioxidant, Scavenging, Hydroxyl radical