Expression of cyclin D1 and p27~(kip1) in renal cell carcinoma and its significance

Objective:To investigate the role of cyclin D1 and p27kip1 in the occurrence and development of conventional renal cell carcinoma(CRCC).Methods: RT-PCR and Western-blot were used to detect mRNA and protein contents of cyclin D1 and p27kip1 in 25 CRCCs and 10 normal renal tissue distant to tumor.Immunohistochemistry was used to investigate the expression of cyclin D1 and p27kip1 in pathological tissue sections of 76 CRCCs.The relationship between those index and clinicopathological parameters was analyzed statistically.Results: In CRCC,the expression of cyclin D1 was higher than that of the control group.The higher cyclin D1 content was related to big tumor size(P0.05);The expression of p27kip1was lower than that of the control group,and the lower p27kip1was related to higher nuclear grade and TNM stage(P0.01).The 5-year cumulative survival rate of the p27kip1high expression group is longer than that of the low group(P0.01).Conclusions: The excessive expression of cyclin D1 and lower expression of p27kip1play an important role in the carcinogenesis of CRCC.The lower expression of p27kip1 may affect the progression of the tumors.The detection of p27kip1 may be as a reference marker in the prognosis of CRCC.