An Experimental Study on the Effects of YISHEN Decoction on the Function and Pathologic Changes of Remnant Kidney after 5/6 Nephrectomy

Objective TO study the effects of YISHEN decoction on the functions and pathologic changes of remnant kidney. Methods Fifty-six rats were divided into normal control group (n=8),model group (n=32) and therapy group with YISHEN decoction (n=32),then the rats of every group were divided randomly into 5weeks,6weeks,7weeks,8weeks groups. The rats of model group and treatment group with YISHEN decoction were operated for 5/6 nephrectomy. Then the rats of model group were fed with Normal Saline ,while those of treatment group with YISHEN decoction (0.3ml/100g/d),once a day,by gavage.The rats of normal control group were fed with normal food and water. All of the rats were killed separately at 5weeks,6weeks,7weeks,8weeks afterwords. The morphologica changes were studied by PAS staining.and the level of serum BUN and Cr. were determined.Rusults In model group, cells in nephridium glomerulus were increased evidently,with more extracelluar matrix (ECM) and crescent shaping.Mesangial section was enlarged and dyed darkly,capillary vessel was open badly or even obturated. In therapy group.cell hyperplasia were not so evident as that in model group. Compared with those of the normal control group and the treatment group , the kidneys of the model group had more severe glomerulosclerosist.Conclusion YISHEN decoction can postpone the process of glomerulosclerosis and kidney function exasperating.