Effect of "Yishen Decoction" on expressions of TGF-β_1 and Collagen III in rats of renal interstitial fibrosis

Objective To investigate the possible preventive mechanism of in the relief of renal interstitial fibrosis in terms of expressions of TGF-β1 and ColⅢ in rats.Methods Thirty-two male SD rats were randomly divided into 4 groups: normal group,model group,Yishen Decoction group and Enalapril group.The models of renal interstitial fibrosis were induced by intragastric administration of adenine.At the end of 1st,2nd and 3rd weeks,the serum Scr and BUN levels were checked;then the rats were sacrificed and the renal tissues were taken for pathological examination by HE staining and Masson staining;immunohistochemistry was used to measure the expressions of TGF-β1 and collagen Ⅲ(ColⅢ) in nephridial tissue.Results In the model group,Yishen Decoction group and Enalapril group there were kidney lesions in different degrees as compared with the normal group.The expressions of TGF-β1 and collagen Ⅲ(ColⅢ) in Yishen Decoction group was lower than those in the model group(P0.05);and Yishen Decoction could significantly decrease the serum levels of BUN and Scr.Conclusion can relieve renal interstitial fibrosis by decreasing TGF-β1 and ColⅢ levels and reducing ECM deposit.