2012•Xiandai shengwu yixue jinzhanRequires access
Effects of Diallyl Disulfide on Apoptosis of Human Leukemia k562 Cells and the Expression of Bax and Bag-1
Yanhui Peng
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Abstract
Objective: To study the effects of diallyl dsulfide(DADS) on apoptosis of human leukemia K562 cells and possible mechanisms.Methods: The morphologic changes of leukemia K562 after DADS treatment were observed by AO/EB.DNA ladder was showed by agarose gelelectrophoresis.Bax,Bag-1 mRNA expression was estimated by reverse transcriptionpolymerase chain reaction(RT-PCR) 48 hours after DADS treatment.Results: The characteristics of apoptosis in k562 cells induced by DADS were observed.The leukemia K562 was treated with 40mg/L DADS for 48 hours reveal an obvious DNA ladder.Expression of Bax mRNA increased,while Bag-1 mRNA expression decreased significantly after DADS treatment for 48 hours compared with the control group(P0.05).Conclusion: DADS can induce apoptosis of human leukemia K562 cells in a concent ration-dependent manner,possilbly through increasing Bax ex-pression and decreasing Bag-1 expression.