Microsurgical Technique of the Model of Rat Orthotopic Liver Transplantation in Three Different Inter-species

Objective To investigate the microsurgical technique of establishing the model of rat orthotopic liver transplantation in three different inter-species/Methods The models of the Wistar、SD and Buffalo rat were established with modified Kamada's two-cuff technique. We monitored the survival rate.Results Three hundred and sixty cases of rat orthotopic liver transplantation were performed in total and the successful rate of the mode was 88.3%. The Wistar、SD and Buffalo group of the survival rate of one week are respectively 91.3%、90.6% and 83.7%. Their survival rates of one month are respectively 83.7%、82.7% and 72.9%. Conclusions The sophisticated microsurgical technique and the delicate surgical manipulation are the key to perform a successful rat orthotopic liver transplantation operation. Tolerance to the operation of pure bred rat is different from hybrid. Operational difficulty due to anatomy variation can be overcome by delicate surgical manipulation.