Study on Endogenous Amino Acid Losses of Broilers by Nitrogen-free Diet Method and Fasting Method

The aim of this paper was to determine endogenous amino acid losses in broilers by Nitrogen-free diet method(NFD)and fasting method(FAS)and compare the difference of the two methods.Twenty 49-day-age healthy AA broiler chickens were divided into two groups randomly,ten broilers each group.The broilers were fasted 48 h and the endogenous amino acid losses were detected by collecting all excrement in 48 h.The results showed that endogenous arginie,threonine,cystine,tyrosine losses determined by NFD were similar to those determined by FAS(P0.05).The other endogenous amino acid losses determined by NFD were extremely significantly(P0.01)or significantly(P0.05)higher than those of FAS.Endogenous sum amino acid losses of broilers by NFD was extremely significantly higher than that of FAS(P0.01).