Expression differential and biological significance of SM22mRNA in gastric cancer and normal tissues

Objective To investigate the relationship between the expression level of SM22(Smooth muscle 22)mRNA and tumorigenesis of human gastric cancer (HGC). Methods Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect the expression level of SM22 mRNA in 42 gastric cancer tissues and 14 normal ones. Results There were 92.86%(39/42) and 71.43%(10/14) positive expression of SM22 mRNA in gastric cancer tissues and normal ones. There was no significant different of the expression positive ratio between gastric cancer tissues and normal ones(P 0.05). The expression level of SM22 mRNA was 0.86 ± 0.14 and 0.32 ± 0.22, respective, after processed with Semi-quantitative. There was significant different of the expression positive ratio between gastric cancer tissues and normal tissues(P 0.05). Of 14 pairs of gastric cancer (GC), 10 pairs Both primary gastric cancer and adjacent normal tissue could express SM22 mRNA, but the expression of SM22 mRNA was significantly higher than the expression in normal issues, 4 pairs SM22 mRNA were only expressed in gastric cancer tissue and was negative in matched normal tissues. Conclusion The expression of SM22 mRNA was significantly higher in gastric cancer than in controlled normal tissue, which suggest SM22 play an role in carcinogenesis, development of gastric cancer.