Detection and genotyping of hepatitis B virus among practitioners in food and public work in Ningbo

Objective:To investigate the genotype of HBV carriers in employees for food and public work in Ningbo,which will do help to the prevention of HBV.Methods:ELISA was adopted to test HBsAg,HBsAb,HBeAg,HBeAb and HBcAb.GPT was detected by Manual method.HBV DNA was detected by gene chip in people whose HBsAg ang HBeAg is positive.Results:There were three modes among 938 of 15000 workers with positive HBsAg(the positive rate is 6.25%):with positive HBsAg and HBeAb(including positive HBsAg alone);with positive HBsAg,HBeAb,and HBcAb(including positive HBsAg and HBcAb);with positive HBsAg,HBeAg and HBcAb(including positive HBsAg and HBeAg).There were no difference of the rate of HBsAg carrier among different gender and occupations.43 cases with positive HBsAg,HBeAg and HBcAb were identified as four genetypes,the classification rate was 93.5%.Among those 43 cases,20 were genotype B with the rate of 46.5%,21 were genotype C with the rate of 48.8%,1 was genotype D and B/C with the rate of 2.3% respectively.Conclusion:There is still a higher rate of HBV carriers in practitioners with the predominant genotype of B and C,which is consistent with the results from the patients in Ningbo.Genotype C has Poor Prognosis.Datas above suggest that we should strengthen the publicity in practitioners and make them fully aware of the dangers of HBV.The disease should be treated in time to slow the progress of the patients' condition.Genotype D is rare in Han people,so we will study the phenomenon further.