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Determination of Concentration of Fentanyl in Human Plasma by HPLC With UV Detection

Enyou Li

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Abstract

OBJECTIVE:To determine the concentration of fentanyl in human plasma by HPLC with ultraviolet detection METHODS:External standard method was used The eluation of fentanyl was carried out by shim-Pack CLC-ODS(6 0mm×150mm,5μm)column with 0 015mol/L NaH2PO4 acetonitril-H2O(30∶70,v/v)as the mobile phase,and detection wavelength was 195nm RESULTS:Calibration curve for fentanyl was linear in the range of 2 0～100ng/ml(r=0 999) The detection limit was 1 0ng/ml The within-day RSD was (6 50±2 79)%,the between-day RSD Was (6 70±3 04)% The method recovery was (91 70±4 70)% The extraction recovery was (97 38±3 69)% CONCLUSION:This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl

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OBJECTIVE:To determine the concentration of fentanyl in human plasma by HPLC with ultraviolet detection METHODS:External standard method was used The eluation of fentanyl was carried out by shim-Pack CLC-ODS(6 0mm×150mm,5μm)column with 0 015mol/L NaH2PO4 acetonitril-H2O(30∶70,v/v)as the mobile phase,and detection wavelength was 195nm RESULTS:Calibration curve for fentanyl was linear in the range of 2 0～100ng/ml(r=0 999) The detection limit was 1 0ng/ml The within-day RSD was (6 50±2 79)%,the between-day RSD Was (6 70±3 04)% The method recovery was (91 70±4 70)% The extraction recovery was (97 38±3 69)% CONCLUSION:This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl

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Available abstract

OBJECTIVE:To determine the concentration of fentanyl in human plasma by HPLC with ultraviolet detection METHODS:External standard method was used The eluation of fentanyl was carried out by shim-Pack CLC-ODS(6 0mm×150mm,5μm)column with 0 015mol/L NaH2PO4 acetonitril-H2O(30∶70,v/v)as the mobile phase,and detection wavelength was 195nm RESULTS:Calibration curve for fentanyl was linear in the range of 2 0～100ng/ml(r=0 999) The detection limit was 1 0ng/ml The within-day RSD was (6 50±2 79)%,the between-day RSD Was (6 70±3 04)% The method recovery was (91 70±4 70)% The extraction recovery was (97 38±3 69)% CONCLUSION:This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl

Key concepts: Fentanyl, Chromatography, Calibration curve, Detection limit, Human plasma, High-performance liquid chromatography, Chemistry, Linear range

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