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2009•Ningxia Medical JournalRequires access

Determination of concentration of fentanyl in human plasma by HPLC

Hai Ke-ron

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Abstract

Objective To establish a HPLC method for determination of concentration of fentanyl in human plasma.Methods The mobile phase was composed of acetonitril-water(0.0015 mol/LNaH2PO4,pH=5.0) =40∶60,the UV detector wavelength was at =200 nm.Results The detection limit was 1ng/ml,and the calibration curve was linear in the range from 6-300ng/m1.The recovery of fentanyl from human plasma was more than 95%.The daily and intraday and RSD were all less than 10.0%.Conclusion The HPLC method is rapid and accurate for determination of fentanyl in human plasma.

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What this paper is about

Objective To establish a HPLC method for determination of concentration of fentanyl in human plasma.Methods The mobile phase was composed of acetonitril-water(0.0015 mol/LNaH2PO4,pH=5.0) =40∶60,the UV detector wavelength was at =200 nm.Results The detection limit was 1ng/ml,and the calibration curve was linear in the range from 6-300ng/m1.The recovery of fentanyl from human plasma was more than 95%.The daily and intraday and RSD were all less than 10.0%.Conclusion The HPLC method is rapid and accurate for determination of fentanyl in human plasma.

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Available abstract

Objective To establish a HPLC method for determination of concentration of fentanyl in human plasma.Methods The mobile phase was composed of acetonitril-water(0.0015 mol/LNaH2PO4,pH=5.0) =40∶60,the UV detector wavelength was at =200 nm.Results The detection limit was 1ng/ml,and the calibration curve was linear in the range from 6-300ng/m1.The recovery of fentanyl from human plasma was more than 95%.The daily and intraday and RSD were all less than 10.0%.Conclusion The HPLC method is rapid and accurate for determination of fentanyl in human plasma.

Key concepts: Fentanyl, Human plasma, Chromatography, Calibration curve, High-performance liquid chromatography, Medicine, Plasma concentration, Detection limit

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