The detection and clinical application of HBV large protein(HBV-LP) in diagnosing viral replication

Objective To explore the clinical significance of HBV large protein(HBV-LP) in diagnosing viral replication,we detected the HBV-LP,HBV DNA and the hepatitis B viral markers(HBV M) in the serum of the patients infected with HBV.Methods HBV-LP and HBV M were analyzed by using ELISA.HBV DNA was quantitatively detected using real-time quantitative PCR.Results 1.No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the same HBV M(P0.05).2.No significant difference between the positive rate of HBV DNA and HBV-LP was found in HBeAg negative patients.There was significant difference between the detectable rate of HBV DNA and HBV preS1 was found in HBeAg negative patients.3.The contents of serum HBV-LP was positively correlated with HBV DNA copies(r=0.986).Conclusion There is a perfect correlation between the positive rate of HBV-LP and HBV DNA,and HBV-LP is a reliable serological marker that can reflect the replication of HBV.