MICROPROPAGATION OF SYZYGIUM CUMINII L.USING IN VITRO TECHNIQUE

Immature seeds of Syzygium cuminii L. produced seedlings after a week when cultured on Murashige and Skoog’s medium supplemented with a cytokinin BA (0.05-1.0 mg/l) and NAA (0.01-0.05 mg/l). The seedling explants were induced to form callus on MS medium containing 2,4-D ( 0.05-1.0 mg/l ) with BA ( 0.5-2.0 mg/l). Such callii subsequently, produced shoot buds when sub cultured on MS medium supplemented with BAP (0.5-2.0 mg/l) singly or in combination with NAA (0.05-0.2 mg/l). Upto 85% of cultures showed shoot formation with about 25 shoots / culture from callus cultured on medium containing 1.0 mg/l of BA along with 0.05 mg/l of NAA. Adventitious shoots showed root initiation when sub cultured on MS medium containing IBA and / or NAA 1.0 mg/l). However further growth and development of rooted shoots occurred only when plantlets were transferred to MS basal medium. About 75% of rooted shoots have successfully been established to soil.