A Study on Tissue Culture of Magnolia officinlis Shoots

By orthogonal test and single factor randomized block design,the best medium combinations for the tissue culture of Magnolia seedlings were investigated inclucling those for the stage of bud inducing culture,proliferation culture,rooting,seedling cultivation and transplanting hardening by means of range analysis, variance analysis and LSD multiple comparison. The test showed: the combination of B5,6- BA 5. 0 mg/L, NAA 1. 0 mg / L+ 2,4-D 1. 0 mg / L was the best for bud induction; the combination of B5,6- BA 5. 0 mg/L+ NAA 0. 1 mg / L + sucrose 15 g / L was the best for Proliferation culture; shoots grew robustly in the medium combination of B5+ NAA 0. 5 mg / L+ GA30. 3 mg / L; besides,the rooting rate was the higest—62. 7% in the medium of B5+ IBA 1. 0 mg / L+ NAA 1. 0 mg / L; ABT1 ABT3could also induce rooting,but the rooting rate was in the combination of B5+ ABT31. 5 mg / L+ ucrose 20 g / L was 1. 5% less than the higest. The transplanting survival rate was 92. 2% when the seedlings were treated with closed bottle hardening 6 days and corkage 6 days in natural light; the survival rate was up to 86% when tansplanting matrix was a 1 ∶ 1 mixture of sand and garden soil. The study will provide technical and theoretical basis for its tissue culture of seedlings.