Diagnostic Value of Lymphocyte CD_(69) Expression for Neonatal Infectious Diseases

Objective To explore the diagnostic value of peripheral blood lymphocyte surface marker CD69 expression for neonatal infectious disease.Methods From Mar.2007 to Dec.2008,82 neonatal patients(including NICU patients) were divided into 3 groups:sepsic infection group(42 sepsic cases),mild infection group(18 mild infectious cases) and non-infection group(22 non-infection cases).The le-vels of lymphocyte CD69 were checked with flow cytometry.SPSS 10.0 software was used to analyze these data.Results The percentage of CD69 expression was(1.13±0.69)% in non-infection group,(2.47±1.03)% in mild infection group,(2.16±1.24)% in sepsic infection group.The percentage of CD69 expression in mild infection group was significantly higher than that in non-infection group(P=0.003),and that in sepsic infection group was significantly higher than that in non-infection group(P=0.017).There was no significant difference between sepsic infection group and mild infection group(P=0.546).Receiver operating characteristic(ROC) curve was used to calculate the critical value.When the critical value of CD69 was 2.19%,the sensitivity of neonatal sepsis was 42% and the specificity was 100%;when the critical value of CD69 was 1.29%,the sensitivity was 83%,and specificity was 56%;when the critical value of CD69 was 0.99%,the sensitivity was 92% and the specificity was 56%.Conclusion Peripheral blood lymphocyte increase significantly in infectious diseases,and CD69 expre-ssion is a good laboratory mark to early diagnosis of neonatal infection.