Genotyping of group A rotavirus in infants and young children with diarrhea in Hohhot

Objective To investigate epidemic characteristics and genotypes of group A human rotavirus in infants and young children with diarrhea in Hohhot from June 2008 to May 2010.Methods Totally 730 stool specimens were collected from children under 5 years old and with diarrhea.Enzyme-1inked immunosorbent assay(ELISA)was used to detect rotavirus antigen.The ELISA positive samples were detected with nest reverse transcription-polymerase chain reaction(RT-PCR) to determine the G and P genotypes of rotavirus.Results Among the samples,298 were rotavirus antigen positive(40.8%);292 were G genotype positive(98.0%);254 were P genotype positive(85.2%);and 250 samples had G and P genotypes(83.9%).Conclusion G1,G3 and P[8] were the predominant genotypes and G1+G3 was the predominant mixture subtype for the epidemic of group A rotavirus in Hohhot and there was a drift of predominant genotype from G1 to G3 from 2008 to 2010.