Application of RAPD to identification and genetic analysis of oil sunflower inbred lines

The identification and genetic analysis of 8 oil sunflower inbred lines were carried out by using RAPD markers.12 effective primers were selected from 40 arbitrary,10-mer primers,and according to the RAPD markers produced by 3 of these primers,all the inbred lines could be identified respectively.of the 12 primers tested against the 8 genotypes,a total of 41 bands were amplified,among which 39 (95.1%) were polymorphic.The products amplified by each primer ranged from1～7,with an average of 3. 4. The genetic distance between two arbitrary inbred lines ranged from 0.13～0.85,with an average of 0.42. The 8 inbred lines could be divided into 2 groups by using UPGMA cluster analysis,and each group could be divided into 2 subgroups.